Modern analytical ultracentrifugation in protein science: A tutorial review
نویسندگان
چکیده
منابع مشابه
Modern analytical ultracentrifugation in protein science: a tutorial review.
Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins. Monitoring the sedimentation of macromolecules in the centrifugal field allows their hydrodynamic and thermodynamic characterization in solution, without interaction with any matrix or surface. The combination of new instrumentation and powerful computational software for data analysis has led to ma...
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Analytical ultracentrifugation (AU) is re-emerging as a versatile tool for the study of proteins. Monitoring the sedimentation of macromolecules in the centrifugal field allows their hydrodynamic and thermodynamic characterization in solution, without any interaction with any matrix or surface. The combination of new instrumentation and powerful computational software for data analysis has led ...
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The reasons for replacing the classical strictly two-component velocity and equilibrium analytical ultracentrifugation Svedberg equations by multicomponent equations, applicable in the study of biological macromolecular systems, are given.
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Methods for quantitative characterization of heterologous protein-protein interactions by means of analytical ultracentrifugation (AUC) include sedimentation equilibrium, tracer sedimentation equilibrium, sedimentation velocity, and analytical band sedimentation. Fundamental principles governing the behavior of macromolecules in a centrifugal field are summarized, and the application of these p...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368
DOI: 10.1110/ps.0207702